DEPARTMENT OF ANIMAL SCIENCE

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Subject: search.filters.subject.Genetic variation

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    BIOCHEMICAL CHARACTERISATION OF MUSCOVY AND MALLARD DUCKS IN NIGERIA
    (2014) Akinyemi, M. O.; Aina, A. J.; Ewuola, E.O.; Osaiyuwu, O.H.; Ajao, E.O.
    Protein polymorphism and alleleic variation were investigated in the blood and egg proteins of the Muscovy and Mallard duck breeds found in Southwestern Nigeria. Four proteins namely haemoglobin, transferrin, carbonic anhydrase, and albumin in the blood and two egg white proteins:ovalbumin and conalbumin were analysed .A total of 50 ducks comprising of 30 Mallard duck and 20 Muscovy ducks and 20 eggs from each breed were used for the analysis. Separation of blood and egg protein genotypes was achieved using cellulose acetate electrophoresis. Two co-dominant allele A and B controlling three genotypes AA, AB and BB were observed at haemoglobin, transferrin and carbonic anhydrase loci investigated for both breeds. A third allele C was observed at the albumin locus in the Mallard breed. . Allele A was the most predominant at the Hb, Tf and CA locus in the Muscovy breed with frequencies 0.775, 0.575, and 0.675 respectively and in the Mallard at the Tf, CA and Al locus with frequencies 0.667, 0.567 and 0.767 respectively. The frequencies of allele A and B at the conalbumin locus were 0.425, 0.575; 0.525, 0.475 in the Muscovy breed. Estimates of heterozygosity were 0.428 and 0.430 in the Muscovy and Mallard ducks respectively. Dendogram generated from the genetic distance values revealed that the two breeds are closely related. High genetic similarity was observed between the two breeds.
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    Genetic Variation In Indigenous Turkey Populations In South West Nigeria
    (2017) Fatai, R.B.; Akinyemi, M.O.; Osaiyuwu,O. H.
    The study was carried out to assess the genetic variation in indigenous turkey populations in South West Nigeria using seven protein markers: Hemoglobin (Hb), Albumin (Alb), Transferin (Tf), Esterase-1 (Es-1), Alkaline phosphatase (ALP), Carbonic anhydrase (CA), and Esterase-3 (Es-3).. A total of 97 turkey birds from Ibadan, Ijebu Ode and Akure were purposively sampled. Separation of plasma and red cell proteins were determined on cellulose acetate electrophoresis to estimate the genetic variability within and among the populations. The degree of heterozygosity, deviation from Hardy Weinberg’s Equilibrum (HWE), FIS, and FST values were estimated. From a total of 7 loci analyzed, six were found to be polymorphic in all the populations sampled while Es-1 was monomorphic. Two codominant alleles which controlled three different genotypes were observed at each polymorphic loci. Deviations from Hardy Weinberg equilibrium and a deficiency of heterozygotes were observed in all the populations. Average heterozygosities ranged between 0.13 and 0.20, heterozygote deficit FIT was estimated at 0.5784 while within breed deficit as evaluated by FIS ranged from 0.38 to 0.56. The fixation index FST revealed that genetic diversity within the studied population was moderately differentiated. Genetic distance among the populations quantified through calculation of Nei genetic distance ranged from 0.037 - 0.109. The result obtained may be used as an initial guide in defining objectives for future investigations of genetic integrity and developing conservation strategies for Nigeria indigenous turkeys.
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    Analysis Of Genetic Structure Of West African Dwarf Goats By Allozyme Markers
    (2016) Awobajo, O.K.; Salako, A.E.; Akinyemi, M.O.; Osaiyuwu, O.H.
    Genetic variation at three allozyme (Haemoglobin, Carbonic Anhydrase and Transferrin) loci and popula-tion structure hypothesis were examined for West African Dwarf goat populations in four Southwesternstate of Nigeria. One hundred and forty animals, twenty from each sampling area comprising Ijebu-Ode and Ado-Odo, (Ogun state), Ondo, (Ondo state), Ile–Ife, Osogbo and Iwo, (Osun state), and Ibadan,(Oyo state), were randomly selected. Estimates of genetic variability such as effective number of allelesand gene diversities revealed substantial genetic variation frequently displayed by allozyme markers. Numbers of alleles observed across the allozyme loci varied from 246 to 250 with an overall meanof 247.33 ± 2.31. Average polymorphism across the studied loci and expected gene diversity in thepopulation were 0.63 ± 0.09 and 0.44 ± 0.09, respectively. Population was observed to be significantlydifferentiated into different groups, and showed fairly high level of outbreeding (f = −0.16 ± 0.44) andexcess heterozygosity
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    Analysis Of Genetic Structure Of Nigerian West African Dwarf Goats By Microsatellite Markers markers
    (2015) Awobajo, O.K.; Salako, A.E.; Osaiyuwu, O.H.
    Genetic variation at 12 microsatellite loci and population structure hypothesis was examined for West African Dwarf (WAD) goat populations found in four Southwestern states in Nigeria. Estimates of genetic variability such as effective number of alleles and gene diversities revealed substantial genetic variation frequently displayed by microsatellite markers. Number of alleles observed across the microsatellite loci varied from 6 to 17 with an overall mean of 10.75 ± 3.22. Average polymorphism across the studied loci and expected gene diversity in the population were 1.66 ± 0.49 and 0.72 ± 0.17, respectively. Population was observed to be significantly differentiated into different groups, and showed fairly high level of inbreeding (f = 0.95 ± 0.15) and global heterozygote deficit.