Chemopreventive effect of ethanolic extract of azadiractha indica on experimental trypanosoma brucei induced oxidative stress in dogs

Abstract

The medicinal properties of Azadirachta indica have boen harnessed for many years in the treatment of many diseases in both humans and animals. Materials and Methods: Twenty-five apparently healthy dogs weighing between 3 and 8 kg were randomly divided into five groups with five dogs in each group. Ameliorative effect of A. indica on erythrocyte anpioxidant status and markers of oxidative stress were assessed. Liver artd kidney function tests were also performed. Results: Pre-treatment with methanolic extract af Azadirachta indica IMEA at different doses did not significantly alter the values of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activity in Trypanosoma brucel infection. Although, serum creatinine significantly (P < 0.05) decreased with pre-treatment with 50 mg/kg A. indice, after 2 weeks of 7. brucelintection. However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively. Markers of oxidative stress such as malond aldehyde and hydrogen peroxide generated were higher in animals infected with 7. brucel with no significant (P>0.051 difference compared to the values obtained in pre-treated animals. Pre treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with 7. brucel. Conclusion: From this study. MEAI showed significant ability to attenuate oxidative stress and inflammation during experimental T. brucei infection.